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Mechanistic interpretations in these studies typically rely on indirect evidence, such as changes in protein markers (e.g., Bax/Bcl-2 ratio, caspase activity), while direct causal validationthrough gene silencing, overexpression, or target binding assaysis rare (Xu G

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Another possibility is that the target structures of non-native IgG in the aggregates may change with increasing particle size

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Briefly, coronal brain sections were immersed in a 1:9 (v/v) solution of 1% sodium hydroxide and 70% ethanol for five minutes, followed by a two-minute wash in 70% ethanol