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The protein complexes that control cell-cell attachment also polarize cellular membrane, so that it can be divided into luminal (blood-facing) and abluminal (brain) sides, and each solute that enters/leaves the brain must cross both membranes

10.1097/md.0000000000038736 170 RinninellaE.CintoniM.RaoulP.GasbarriniA.MeleM

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Cycling conditions were as follows: 30 seconds at 95 C, then 40 cycles of 10 seconds at 95 C, 30 seconds at 60 C followed by a melt curve from 65 C to 95 C at 0.5 C intervals