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In order to interpret the observed changes, a conformational search was performed and the ECD spectra were calculated for the 20 lowestenergy conformers, and those that contribute to the observed ECD spectra with an increased probability were identified
E2E1-I53-50A contained elevated levels of oligomannose at positions 430, 448, 476, and 623 possibly reflecting steric occlusion of these sites to mannosidase because of the trimeric organization of E2E1
OSullivan et al 2014)
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Comprehensive evaluation helps identify contributing factors that need direct treatment
