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doi: 10.1152/ajpgi.00001.2006
[DOI] [PMC free article] [PubMed] [Google Scholar] [10].Guarnieri JW, Angelin A, Murdock DG, Schaefer P, Portluri P, Lie T, et al
The samples were subsequently reduced with 4 mM DTT for 30 min at room temperature with shaking at 1,000 rpm, followed by alkylation with 10 mM iodoacetamide for 45 min in the dark at room temperature while shaking at 1,000 rpm

discussion 290-293

According to the data presented above, we propose the following optimal dosing (Table 1)

All cited studies are conducted in cellular or rodent systems
