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The total cell lysate was cleared by centrifugation and supplemented with 25 L per liter culture OD 600 of BioLock avidin (IBA Lifesciences, Gttingen, Germany) to mask D-biotin from the culture medium and/or endogenous biotinylated proteins from the host cell, followed by sterile-filtration

10.1038/s41574-018-0059-4 Nat Rev Endocrinol

In a recent OVX-rat study (22), quercetin reversed the elevation of Bacilliota and reduction of Bacteroidota , significantly lowering the F/B ratio which is commonly associated with intestinal health and metabolic disorders, with a lower ratio favoring increased synthesis and secretion of SCFAs by intestinal flora, and enriching SCFA-producing taxa (42)

& Yui, N

Visual inspection showed acceptable symmetry, with no major deviation from the vertical axis, which was further confirmed by the non-significant results of Eggers test as detailed in the summary

A: Formal analysis, Investigation, Methodology, Writing original draft