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For detection of pimonidazole, the tumor sections were treated with mouse anti-pimonidazole primary antibody (dilution 1:200, Hypoxyprobe Inc.) and Alex 488-conjugated goat anti-mouse secondary antibody (dilution 1:200, Jackson Inc.) following the kits instructions

Patients with pre-existing gastrointestinal conditionsinflammatory bowel disease, irritable bowel syndrome, or previous bowel surgeryshould maintain closer monitoring and lower thresholds for seeking medical input, as these populations may experience more pronounced or complex digestive effects

International sourcing cannot offer that timeline or that temperature reliability for compounds this sensitive

Further studies to identify the clear mechanisms of KD and drug interactions, different KD responses in heterogeneous patient backgrounds, and clear mechanisms of KD intervention in the fight against numerous specific central nervous system diseases are crucial

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