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After incubation at 37 C for 1 h, the media were removed and formazan crystals in the cells were dissolved in 1 mL DMSO and processed for OD reading at 570 nm using a spectrophotometer

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What are the effects of vitamin B12 deficiency in horses: These include weight loss, diarrhea, low energy levels, teeth issues, a dull coat, hoof troubles, parasites or an increased susceptibility to illness

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