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Western blotting of separating nuclear and cytoplasmic extracts revealed a significant increase in FOXO4 expression in H 2 O 2 -induced senescent TM3 Leydig cells, and confirmed that FOXO4 protein was concentrated in the nucleus (Figure 3C3E)

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Age-related decline in melatonin contributes to enhanced osteoclastogenesis via disruption of redox homeostasis

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Only when insulin was given back to where it was missing, the portal vein, did serum IGF-1 levels normalize