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The mechanism comprises a sequence of reactions with first 1acyl-DHAP binding to AGPS, then reaction of acyl-DHAP with the cofactor flavin adenine dinucleotide (FAD) for release of a fatty acid containing both oxygen atoms from the ester linkage in acyl-DHAP, binding of the fatty alcohol to AGPS, and finally formation of 1alkyldihydroxyacetone phosphate (1alkyl-DHAP)

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