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Carbon-based NMs offer multiple advantages in MALDI-MS analysis, including: (1) enhancing D/I efficiency and sensitivity, reducing background noise, and expanding applications to small molecules, (2) serving as efficient adsorbents and, when combined with their high performance in MALDI-MS to improve analytical sensitivity, and (3) further improving detection selectivity through the functionalization of carbon-based NMs

Among tryptophan metabolism pathways, 5-hydroxytryptamine (5-HT) is a monoamine produced by the essential amino acid tryptophan (73) and participates in the regulation of polyamines

GSH deficiency can interfere with the body's ability to detoxify the cellular microenvironment, fold proteins, replenish antioxidants, maintain healthy immune responses and even modulate apoptotic events, promoting suboptimal cellular function and development of diseases [53]