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The mixture was monitored for absorbance changes every 30 or 60 s for two minutes at 560 nm [31]

Quantitative PCR Viral RNA copies were determined by quantitative PCR (qPCR)

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When poured into a secondary container (i.e., basin), Revital-Ox RESERT HLD solution can be reused for up to 21 days or until its concentration falls below the minimum recommended concentration of 1.5% hydrogen peroxide