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We then found that TfR1 staining could be coupled with machine learning to distinguish cells undergoing ferroptosis from those undergoing apoptosis (Jin et al., 2022)

By modeling gene deletion and predicting growth, this method can forecast the impact of the loss of individual metabolic routes on growth before experiments and help identify candidates of attenuation ( aroA and thyA ) to ensure that the engineered bacteria are safe in normal tissues but remain active in the TME (110)

This shift results in lipid peroxide accumulation, ultimately triggering ferroptosis

And that is where the paradigm shift began