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Samples were transferred onto a nitrocellulose membrane (0.2 mm, Hybond ECL, Amersham Italia) by a wet transfer unit (Mini Blot Module, Life Technologies)

Non-invasive human biomarkers and imaging In people, liver biopsy with histologic scoring remains the regulatory gold standard, but it is invasive, subject to sampling variability, and impractical for screening or repeated monitoring

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Discussion 5.1

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