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Pellets were resuspended in 40 L lysis buffer (TBS containing 0.7% triton-X, 0.1% tween-20, and 1 Halt Protease and Phosphatase Inhibitor (PPI) Cocktail (ThermoScientific 78440)) and lysed on ice for 20 min

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[DOI] [PubMed] [Google Scholar] 111.Lee NC, Tang NL, Chien YH, Chen CA, Lin SJ, Chiu PC, Huang AC, Hwu WL

Hence, the overall stoichiometry for the synthesis of palmitate is Palmitate produced by fatty acid synthase can generate other longer fatty acids by elongases, which lengthen palmitate or undergo desaturation by desaturases to generate unsaturated fatty acids

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