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Russell RM, Baik H, Kehayias JJ

Supernatants were collected and the protein concentrations were determined using a BCA assay kit (Pierce/Thermo Scientific, Rockford, IL) for Western blot analysis and high performance liquid chromatography (HPLC) assay

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Cell cultures, preparation of peritoneal macrophages, cell proliferation and cell stimulation The murine macrophage cell line RAW 264.7 cells were purchased from Korea Cell Line Bank (KCLB, Seoul, Korea)

Hopefully, in the light of the suggested stomach significance for bone homeostasis, the possible relevance of this pentadecapeptide BPC-157 effect (local or intramuscular effectiveness, lack of unwanted effects) could be a basis for methods of choice in the future management of healing impairment in humans, and requires further investigation