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[PMC free article: PMC508574] [PubMed: 9435307] 36

Handling: Reconstitution and storage should follow standard laboratory protocols
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Notably, post-mortem studies revealed reduced activity of respiratory chain complexes in a group of ASD children, suggesting that abnormalities, especially in the frontal, temporal and cerebellar areas (Chauhan et al., 2011
The reaction mixtures were incubated at 37 C for 30 min prior to the addition of 15 l of malachite green reagent
