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Tissues were homogenized in 510 mL of cold buffer (50 mM potassium phosphate, pH 7.5, 1 mM EDTA) per gram of tissue, followed by centrifugation at 10,000 g for 15 min at 4 C

however, it can be considered as safe

11 When HSP90 function is inhibited, the AR protein misfolds due to the loss of chaperone protection and is eventually degraded by the ubiquitinproteasome system, thereby blocking the AR-dependent transcriptional program

AG and DF provided the mouse model and performed the in vivo experiments

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