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Shorb knew that the same liver extract was used to treat pernicious anemia (her father-in-law had died from the disease), and concluded that LLD could be developed as an assay method to identify the active compound

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This sudden cellular proliferation leads to massive potassium uptake by newly forming cells, causing a dramatic shift of potassium from outside the cells to inside them

That review also found moderate evidence that treatment with vitamin B12 does not improve dementia and that oral supplements are as effective as injections of vitamin B12

In research settings, it is investigated for its interactions with angiogenic and inflammatory pathways, making it a subject of interest in studies on cellular response and recovery models