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In the study, the cell viability of human colon cancer cells decreased to 7.0 percent in 48 hours, suggesting that the remaining 93 percent of the Caco-2 cells were killed by the dose of lauric acid applied to them

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Formalin-fixed liver samples were cryoprotected in 20% sucrose at 4 C overnight, blotted, then liquid-nitrogen snap frozen in OCT compound (Tissue-Tek) and stored at 80 C

Protein bands of interest were excised and subjected to in-gel digestion as described above

Moreover, an increasing number of biological functions are now known to involve glutathione, such as that in the biosynthesis of iron-sulphur clusters of mitochondrial enzymes [32,33]

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