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These are relied upon by some of Californias most vulnerable populations, including chronic illness sufferers, cancer patients and firefighters

In EcGGT, replacement of the second and third residue, respectively, with alanine (T392A) and glycine (H393G) impaired autoprocessing, suggesting that the small subunit N-terminus is more crucial than the large subunit C-terminus during autoprocessing (Hashimoto et al., 1995)

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For RNAi experiments, eggs were isolated by treating adult hermaphrodites with alkaline hypochlorite and allowed to develop and grow for two generations on specific RNAi expressing bacterial strains before being used or lifespan analyses