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McIlwain, D

753483) and were maintained at 37 C in a humidified incubator with 5% CO 2

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Instead of improving dissolution, vigorous agitation may: Reduce molecular stability Promote aggregation Generate foam Compromise experimental consistency Best Practice Rather than shaking the vial: Allow the solvent to work naturally

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With a dielectric constant of 6.2, it effectively dissolves both inorganic salts and non-polar compounds, helping peptides that are otherwise difficult to reconstitute