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Description
studied the sensitivity of tumor cells to mitochondrial ETC inhibitors and found that tumor cells insensitive to ETC inhibition maintain intracellular Asp concentrations through the Asp/glutamate transporter SLC1A3, which gives tumor cells a survival advantage

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Permeabilize the cells with 0.25% Triton X-100 in TBST(Tris buffered saline with Tween) for 10 minutes 5

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