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In mitapivat-treated 4.2 / mice, we noted decreased protein oxidation in liver as determined by Oxyblot (Figure 5C and Supplemental Figure 8B), which was accompanied by blunted activation of Nrf2 and NF-B p65 (Figure 5D and Supplemental Figure 8C) with resultant downregulation of Nrf2-related cytoprotectors such as heme-oxygenase-1 (HO-1) and Gpx1 (Figure 5E and Supplemental Figure 8D) (17, 46, 47)

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