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The crosslinked proteins were digested and analyzed by tandem mass spectrometry to identify crosslinked peptides (Supplementary Data 1)

Specifically, the genes required for module II and module III were cloned into plasmid pCS27 and transferred into strain BW25113 (BW, for short), generating strains BW3 (BW/pCS-LsBAHD-panE), BW4 (BW/pCS-LsBAHD-ScAAE-Scgloxdh) and BW5 (BW/pCS-LsBAHD-ScAAE-Fpoah), respectively

Lipid mixtures were prepared and added to a chamber built of ITO-coated slides (Przisions Glas & Optik) which were glued together with Vitrex (Vitrex Medical)

Biochemistry 34 , 1540715414 (1995)

With the exception of dominant follicles, which are released for fertilization, the other growing follicles all undergo apoptosis, and this process is promoted by ROS