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This still requires further investigation of the clinical significance of glycosylated albumin in the pathophysiology of liver disease
They were transfected for 3 h in Opti-MEM I (Invitrogen corp., carlsbad, CA, USA), and then the medium was changed to RPMI 1640 with 5% fetal bovine serum and they were incubated for further 33 h

[DOI] [PMC free article] [PubMed] [Google Scholar] 73.van Langen M.L., Hotte N., Dieleman L.A., Albert E., Mulder C., Madsen K.L

The additional vitamins enhance the overall effect
