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After obtaining the cell wall samples, the chitin content was measured using the Chitin ELISA Kit (Shanghai Enzyme-linked Biotechnology Co., Ltd., Shanghai, China) according to the manufacturers instructions

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4 [104] Who should be tested for Lp(a) and when The most recent guidelines from the European Atherosclerosis Society (EAS) and the American Heart Association (AHA) recommend that every adult should have their Lp(a) levels determined at least once in their lifetime to identify individuals with very high congenital Lp(a) levels, which significantly increase cardiovascular risk [100, 105] (Table II)
