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The Longa method was employed to establish the middle cerebral artery occlusion (MCAO) model in rats 12 : the common carotid artery (CCA), external carotid artery (ECA), and internal carotid artery (ICA) were exposed through a midline incision in the neck

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It may overdrive metabolic processes initially

In reproductive organs, it may reduce oxidative damage by inducing angiogenesis, inhibiting cytokine-driven ROS, and facilitating extracellular matrix repair

Replenishing the nutritional status of the host by increasing vital amino acids such as cysteine to enhance GSH levels and selenium to improve selenium deficiency and facilitate selenoprotein (GSH peroxidases, thioredoxin reductases) expression can inhibit oxidative stress, modulating inflammation, suppressing endothelial dysfunction, and protecting vascular cells against apoptosis and calcification (He et al., 2017

This supports the hypothesis that wave-like propagation of lipid peroxidation, membrane damage and subsequent calcium fluxes do not require complete cell lysis [269]