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In vitro studies confirm minimal cytotoxicity with no impact on cell viability up to 100 M concentrations across multiple cell types, though higher concentrations may produce non-specific effects that could confound experimental results

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These diverse applications provide researchers with validated experimental frameworks for studying senescence contributions to age-related phenotypes across multiple tissue types and organ systems

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Since most ET is exhaled unmetabolized, breath analysis provides the best estimate of total ET exposure