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doi: 10.1096/fj.04-2079rev, PMID: [DOI] [PubMed] [Google Scholar] 101.Rodriguez C, Mayo JC, Sainz RM, Antolin I, Herrera F, Martin V, et al
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Efect of digoxin on the circulation in normal man

Fixative was then removed and neutralized twice with 100 mM Tris Buffered Saline in MilliQ water and then washed once with 0.1% Tween-20 in PBS
The molecular weight cutoff is not absolute, as molecular structure, charge, and lipophilicity also play roles, but it is the most reliable general predictor

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