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Serum-free or low-serum conditions are essential because serum IGFBPs in the media would partially sequester even LR3 at the highest serum concentrations

Oral Supplements Cyanocobalamin tablets (common) Methylcobalamin (easier to absorb) B12 sprays or dissolvable tabs Pros: Easy to take No needles Affordable Cons: Absorption varies Not ideal for GI conditions Food Sources Liver (highly concentrated) Eggs Red meat Cheese Shellfish and fish Plant-based

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Many opt to take supplemental B12 alongside a folate supplement to avoid this risk (23, 24)

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