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After 24 h, mitomycin C (Sigma Aldrich) was added for 2 h to block cell proliferation
[DOI] [PubMed] [Google Scholar] 10.Bus JS, Cagen SZ, Olgaard M, Gibson JE

PMID: 19117343

Suppose we had added the same amount of HCl or NaOH solution to 100 mL of an un buffered solution at pH 3.95 (corresponding to 1.1 10 4 M HCl)

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In vitro pituitary studies demonstrate higher GH release compared to individual peptide exposure