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A complementary axis involves transcriptional reinforcement of antioxidant programs, including NFE2 like BZIP transcription factor 2 (NFE2L2, also known as NRF2) signaling, which increases GPXs and further suppresses ROS-driven inflammasome activation 58

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Glutathione is an antioxidant compound in the cells of nearly every type of organic life on earth

[DOI] [PubMed] [Google Scholar] 26.Bao P., Zheng Z.T., Ye J.J., Zhang X.Z

[DOI] [PMC free article] [PubMed] [Google Scholar] 116.Calvert P., Yao K.S., Hamilton T.C., ODwyer P.J

After 24 h, viability was assessed using the AquaBluer assay as previously described ( Determination of GSH Levels HT-1080 cells were cultured in DMEM containing 10% FBS, 1% supplemented non-essential amino acids and 1% Pen-Strep mixture (all from Gibco), and maintained in a humidified atmosphere with 5% CO 2 at 37C