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The supernatant was removed and total protein concentration in the soluble fraction was determined using a NanoDrop UV spectrometer (Thermo Fisher Scientific) before being transferred to standard chromatography vials with polypropylene inserts (Agilent Technologies) and kept at 4 C on a Peltier cooler for analysis

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Then, MML-CDs with an equivalent concentration of 1 mg/mL were co-incubated in the simulated gastric fluid, simulated intestinal fluid and simulated colonic fluid, respectively

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