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doi: 10.1111/dth.15331

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Gid4 stabilization assay Yeast cells expressing 3HA-GID4, GID12-6HA and 3HA-GID4, pGPD-GID12-6HA were grown in YPD/YPE medium respectively as mentioned in the section Yeast strains and growth conditions

When oxygen is available, pyruvate can be oxidized to acetyl CoA, with a reduction of NAD +

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Pfu high-fidelity DNA polymerase was used for PCR amplification, and the number of cycles was strictly controlled to ensure the same amplification conditions for samples