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The decreased activity of the ALDH2 2*2 variant stems from a single amino acid substitution, where lysine replaces glutamate, resulting in a 200-fold increase in the KM (which represents the affinity of an enzyme for its substrate) for NAD+ binding

Also, this technique is appropriate for investigating the direct interaction between two purified proteins

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Meissner et al., 2015)

It should be noted that there are no guidelines stating the upper limit of MSG in natural food in the literature