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p53 TAD (13-61) was subcloned into the pET His 6 glutathione-S-transferase (GST) TEV LIC cloning vector (2G-T, obtained from Scotta Gradia, Addgene plasmid #29707) and transformed into BL21 (DE3) pLysS cells
Further complicating the process is the wide variability in patch testing protocols, both in the allergen panels offered and in interpretation practices, depending on provider training, geographic region, and clinical resources [70]
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The cells in control well were not treated with FOXO4-DRI, and the blank well containing the medium without cells were used to normalized
