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Having incubated the immunoprecipitated samples overnight at 4 C with rotation, the anti-M2 FLAG antibody-conjugated agarose beads were washed three times (each for 5 min) with ice-cold washing buffer (50 mM Tris, pH 7.4, 137 mM NaCl, 10% glycerol, 1% NP-40)

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Cell 71 (2), 306318.e7

[PubMed] [Google Scholar] 109.Tanaka E, Yamazaki K, Misawa S

ISSN 1474-9718

TMJ disorder refers to a range of conditions that cause pain and dysfunction in this joint and the muscles surrounding it
