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Behavioral testing Rats were acclimatized to the testing environment for 3 consecutive days before baseline testing and were tested for mechanical and thermal hyperalgesia in a blind manner to avoid potential bias

Urea (8 M) was added to the precipitate to dissolve the RIPA-insoluble protein components

Bacteriostatic waters design supports up to 28 days of reliable use from one vial, reducing cost, plastic waste, and preparation time while keeping concentrations consistent across experiments

Across most animal and human studies, two primary routes dominate [1]: Subcutaneous injection : Used in numerous rodent studies (typically 0.110 g/mouse/day) and in human trials (commonly 0.51 mg/day), especially in circadian and immune studies