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These results demonstrated minimal variability and robustness of the data generated from all three platforms (Supplementary Fig

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However, sputums inherent complexity (mucus, cells, bacteria, mediators) challenges assay standardization, necessitating meticulous sample processing to preserve enzyme activity and minimize interference (Dosanjh, 2008)

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Furthermore, the results of HTR8 cells indicated that H/R treatment downregulated the mitochondria marker TOMM20 and upregulated the expression of BNIP3 and the LC3II/LC3I ratio (Figures 5C, D)

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Statins are known to provide many benefits to endothelial function independent of cholesterol levels, many of which involve nitric oxide (NO), a second messenger molecule deeply rooted in signaling pathways related to endothelial functions

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