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Based on these findings, the study authors concluded that vitamin B12 supplementation should not be considered a chemopreventive strategy for lung cancer and may potentially have the opposite effect

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Uptake of CLF To measure CLF polarity, organoids were removed from the Matrigel by washing with PBS and incubated with culture medium supplemented with 10 g/ml CLF (Corning Inc.) and 1 g/ml Hoechst 33342 for 30 minutes at 37 C in a 5% CO 2 incubator

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doi: 10.3389/fnagi.2021.772278