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Description
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It was initially planned to recruit 20 analyzable patients (who completed the two-treatment periods) to have 80% power to detect, with a two-sided test at 0.05 significance level, an absolute decrease in percentage over total target of 10% under the A-dopamine period compared with the control (oral l-dopa-alone treatment) period, by assuming a standard deviation of 15% and a within-patient correlation of 0.5 to account for the crossover design
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Then, 5 L of 200 mM DTT was added and incubated for 40 min at 60C
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The closet genes to the peaks were determined using bedtools 2.29.2 (Quinlan and Hall 2010)
