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However, it remains unclear whether this approach influences the biological activity of peptides and proteins

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A comparative study 11 of 5 empirical pK a prediction tools (ACD/pK a DB ( Epik ( VCC ( Marvin ( and Pallas (www.compudrug.com)) on 248 compounds of the Gold Standard Dataset compiled by Avdeef 12 , demonstrated a tendancy for prediction errors to be higher for compounds with a larger number of possible tautomeric states

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10.3389/fmed.2025.1601806 305 ZhengD.ChenW.ChenT.ChenX.LiangJ.ChenH.et al (2024)

Either way, cars are still getting through just slower