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Importantly, Munc18-1 Q301D expression in Munc18-1 deficient neurons severely reduces synaptic transmission, demonstrating the physiological significance of the Munc18-1VAMP2 interaction.

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Adding a GST tag (purple) to your protein of interest (dark blue) enables GST pulldowns to discover novel interacting partners (orange, green, and light blue)

Conclusion All six injection techniques reliably enter the CT space