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At the age of 5.0 years and 6.3 years, additional diagnoses comprised nutritional problems, speech developmental disorder, and flat, skew feet
Samples were left to solubilize overnight at room temperature before 2X Laemmli was added prior to Western Blot analysis

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We conclude that increased research efforts are warranted regarding the safety and efficacy of GBH as it pertains to female reproduction, as well as investments in cost-effective alternatives with the potential to reduce GBH usage

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doi:10.1016/j.bbagen.2013.04.016 2 Millea, P
