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SDS-PAGE resolves this by denaturing all proteins into rod-shaped SDS-protein complexes with uniform charge density (~1 SDS per 2 amino acids), so that mobility in the polyacrylamide gel is determined solely by the sieving effect of the pore matrix, giving a linear relationship between log molecular weight and relative migration distance (Rf)

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doi: 10.1038/ejcn.2009.4

This has to be scanned onto your notes .I get a prescription to self inject subcutaneously

Tateno, C
