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2.5 Cell culture The Eca-109 cell line of ESCC was grown in DMEM with high glucose, 10% fetal bovine serum, and 1% penicillinstreptomycin solution at 37 C in a 5% carbon dioxide humidified environment

Plateletlymphocyte ratio can be used as an inflammatory marker [29]

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Disruption of Iron Homeostasis in Neurodegenerative Diseases 6.1

Predictably, observed molecular weight of DJ-1 on western blots is lower than anticipated (26 kDa instead of 30 kDa), probably due to cleavage of mitochondrial targeting sequence