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[DOI] [PubMed] [Google Scholar] 106.Ma KH, Lippner DS, Basi KA, et al

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Antigen-specific affinity purification of antibodies involves two processes: antigen immobilization to a solid support and antibody purification procedure from the serum

The total recording should last 6 min, capturing the animals behavior during the experiment, but focusing on the last 4 min of immobility time