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Description
M., Cestari, C., Stabilini, R., Conte, D., Zappal, F., Floreani, A., Montalto, G., Soresi, M., Notarbartolo, A., Buscarini, E., Buscarini, L., Buongiorno, G., Manghisi, O

Lysates of 100 g total protein was used for each assay on a 96-well plate and incubated with 10 M of the test compound for 10 min on ice
the frequency of these reactions is unknown

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l-Leucine influx through Slc7a5 regulates inflammatory responses of human B cells via mammalian target of rapamycin complex 1 signaling

The gradient started at 5% B (held for 2.5 min) and was linearly increased to 30% B within 37.5 min, to 50% B within 10 min, then to 85% B within 5 min (held for 3 min)
