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This is why a reconstituted vial left at room temperature overnight is almost certainly compromised, while an unreconstituted vial in the same scenario retains most of its original potency

Immune Netw (2013) 13(5):2137

Any bacteria that enter the vial can't reproduce

Decreased stomach acid production also provides an environment conducive to the overgrowth of anaerobic bacteria in the stomach, which further interferes with vitamin B 12 absorption (3)

Always refer to established scientific literature or peer-reviewed studies for initial dosage ranges

ebei, B., Nikoli, V., Sikiric, P., Seiwerth, S., oa, T., Patrlj, L., Grabarevi, ., Ruman, R., Petek, M., Konjevoda, P., Jadrijevi, S., Perovi, D., & laj, M