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Elution is usually complete after 35ml of buffer is collected per 1.0ml of settled resin, provided the imidazole concentration is high enough to efficiently elute the protein of interest
Kubota, J
PBS/S was used as blocking buffer, PBS/S/T was used as dilution buffer and PBS/T was used as wash buffer in between each step
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For many companies, this challenge can stem from the approach they take to recruitment, which is often an afterthought
